Plant Regeneration and In Vitro Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, Cryptomeria japonica) Derived from Different Embryogenic Cell Lines

With the spread of pollinosis caused by sugi (Japanese cedar, Cryptomeria japonica) pollen, use pollen-free somatic seedlings is expected in Japan. However, there a lack knowledge on relationship between abilities during embryogenesis, initial vitro growth traits, and subsequent seedlings. In present study, we provide first basic information embryo maturation efficiency, germination, plantlet conversion frequencies, as well performance plantlets derived from different embryogenic cell lines (ECLs). Somatic efficiency varied 34 to 514 cotyledonary embryos per plate average for 19 ECLs tested was 244 plate. Subsequently, overall rates germination among were 87.8% 85.3%, respectively. The results showed significant differences rate ECLs.